The Myakka City Lemur Reserve is located in extreme eastern Manatee County, and is surrounded on all sides by agricultural land. A variety of habitat types occur on the site due to topographical gradients, various soil types and historical land-uses, allowing environmental diversity on the reserve.
The vegetation zones are defined as oak-pine mixed forest, upland coniferous forest, live oak hammock, mixed rangeland, pine flatwoods, scrub and brush land, palmetto prairie, freshwater marsh, and wetland mixed forest.
The lemur enclosures are located in the forests. Every effort is being made to maintain the colony in as natural a state as possible so that behavioral research will be maximally unbiased.